Title : Dillenia indica fruit extract suppressed diet-induced obesity in rat by down-regulating the mRNA level of proadipogenic transcription factors and lipid metabolizing enzymes

Authors : Syed A. Kuddus, Zarin Tasnim, Md. Hasanuzzaman Shohag, Tahmina Yasmin, Md. Sahab Uddin, Muhammad M. Hossain, Nusrat Subhan, Md. Ashraful Alam, Ferdous Khan

Abstract : Background: Dillenia indica (Family: Dilleniaceae) is an antioxidant-rich edible fruit-bearing medicinal plant. The fruit of this plant (known as elephant apple) has many uses in traditional medicine. Objective: By considering its antioxidant content and ameliorating effects this study was aimed to evaluate the antiadipogenic effects of D. indica fruit extract (DIFE) in high-fat diet (HFD) fed obese rats. Methods: Male Wistar rats were fed with a standard diet (SD), or high-fat diet (HFD), or HFD with 100 mg/kg or 200 mg/kg or 400 mg/kg DIFE for 8 weeks. The fruit extract was given orally by feeding gavage. The body weight, liver weight, visceral fat weight, plasma lipids, and oxidative stress-related parameters were measured. The mRNA level of different adipogenesis related transcription factors, lipogenic and lipolytic enzymes was also evaluated. Results: Consumption of DIFE daily (400 mg/kg) for 8 weeks resulted in a significant reduction of high-fat diet-induced body weight, liver weight, visceral fat weight, total cholesterol, and LDL-cholesterol level. High-fat diet-mediated elevation of oxidative stress markers was also lowered, with a parallel augmentation of the activities of antioxidant enzymes, due to 400 mg/kg DIFE feeding. DIFE also down-regulated the mRNA level of important pro-adipogenic factors like PPAR?, LXR?, and SREBP1c which consequently down-regulated the transcript levels of lipogenic enzymes: ACC, FAS, HMHCR, and DGAT. The transcript level of lipolytic enzyme, HSL was also down-regulated in 400 mg/kg DIFE-fed rats. Conclusion: These findings indicate that the antioxidant-rich ethanolic extract of D. indica fruit can down-regulate the gene expression of pro-adipogenic transcription factors and lipid metabolizing enzymes and thus can suppress dietinduced obesity in Wistar rat.

Journal : Current Nutrition & Food Science Volume : 16 Year : 2020 Issue : 1
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