Title : Repeated administration of high dose caffeineinduces oxidative damage of liver inrat: Health and ethical implications

Authors : Nasrin Akhter, Md. Ashraful Alam, Md. Anower Hussain Mian, Hasan Mahmud Reza ,Darryl Macer, Md. Saiful Islam

Abstract : Caffeine, a known CNS stimulant is given as an adjunct component in most abused drugs which could be fatal with repeated administration in many circumstances. This paper presents astudy to investigate the effect of repeated administration of caffeine at high dose on rat liver, and discusses ethical and policy issues of caffeine use. Long Evans rats were treated with pure caffeine solution in distilled water through intragastric route once daily for consecutive 56 days. Three groups of rats recognized as low dose, high dose and control group received 6mg caffeine / kg BW, 12mg caffeine / kg BW and distilled water,respectively. Rat plasma was examined for liver transaminases (ALT, AST) and alkaline phosphatases (ALP) concentrationswhich were significantly increased in plasma as compared tothecontrol. Both rat plasma and liver homogenate were subjected to estimate malondialdehyde (MDA), advanced oxidation protein product (AOPP), nitric oxide (NO), antioxidant enzyme catalase (CAT), glutathione (GSH) and superoxide dismutase (SOD) activity. MDA, AOPP, NO levels increased and SOD activity decreased significantly in both plasma and liver as compared to those of control where as CAT and GSH activity remain unchanged. Rat liver tissues were studied histochemically with Hematoxylin andEosin,and Picro Sirius Red staining. Significantly increased infiltration of inflammatory cells and progressive deposition of collagen fibre were visible in liver tissue of caffeine treated both dosegroups as compared to the control.Long term administration of caffeine at higher dose, significantly contributes to liver inflammation and consequent fibrogenesis.This raises significant ethical and policy issues .

Journal : Eubios Journal of Asian and International Bioethics Volume : 4 Year : July 2018 Issue : 28
Pages : 104-112 City : Edition : Editors :
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