NSU Research Contributions
Title : Effects of Gamma Irradiation on Bacterial Microflora Associated with Human Amniotic Membrane.
Authors : Fahmida Binte Atique, Kazi Tahsin Ahmed, S. M. Asaduzzaman and Kazi Nadim Hasan
Abstract : Human amniotic membrane is considered a promising allograft material for the treatment of ocular surface reconstruction, burns, and other skin defects. In order to avoid the transmission of any diseases, grafts should be perfectly sterile. Twenty-five amniotic sacs were collected to determine the microbiological quality of human amniotic membrane, to analyze the radiation sensitivity pattern of the microorganism, and to detect the radiation decimal reduction dose(D10) values. All the samples were found to be contaminated, and the bioburden was ranged from 3.4×102 to 1.2×105 cfu/g. Initially, a total fifty bacterial isolates were characterized according to their cultural, morphological, and biochemical characteristics and then tested for the radiation sensitivity in an incremental series of radiation doses from 1 to10 KGy. The results depict gradual decline in bioburden with incline of radiation doses. Staphylococcus spp. were the most frequently isolated bacterial contaminant in tissue samples(44%). The ????10 values of the bacterial isolates were ranged from 0.6 to 1.27 KGy. Streptococcusspp. were found to be the highest radioresistant strain with the radiation sterilization dose (RSD) of 11.4 KGy for a bioburden level of 1000. To compare the differences, ????10 values were also calculated by graphical evaluations of the data with two of the representative isolates of each bacterial species which showed no significant variations. Findings of this study indicate that lower radiation dose is quite satisfactory for the sterilization of amniotic membrane grafts. Therefore, these findings would be helpful to predict the efficacy of radiation doses for the processing of amniotic membrane for various purposes.
| Journal : Biomed Research International | Volume : 2013 | Year : 2013 | Issue : |
| Pages : 1 -6 | City : | Edition : | Editors : |
| Publisher : | ISBN : | Book : | Chapter : |
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