Title : Liposomal Drug Delivery System of Corchorus olitorius Leaf Extract Containing Oleic Acid And Phytol

Authors : H. B. Noor, T. Jahan, N. Nawar, M. Kazi, S. R. Lubna, H. M. Reza, M. H. Shariare

Abstract : Purpose Oleic acid and phytol, the main active components of Corchorus olitorius, exhibit a variety of medicinal properties (antioxidant, antibacterial, and antitumor etc.) however, they are hydrophobic. Therefore, the aim of this study was to develop a liposomal formulation of the extract to improve the solubility and for targeted drug delivery. Methods Dried Corchorus olitorius leaves (COE) were macerated for 72 hours using methanol as a solvent. Total phenol content (TPC) and total flavonoid content (TFC) were measured by Folin–Ciocalteu and aluminium chloride colorimetric assay methods, respectively. Phospholipid was extracted in-house from eggs and peanuts, which was quantified using Ultra Performance Liquid Chromatography (UPLC). COE was encapsulated in liposomes using ethanol injection method. A full factorial design considering factors [Process parameters – stirring time, rate of injection, stirring speed and temperature; Material attributes- amount of extract, phospholipid: cholesterol ratio, pH of buffer and amount of drug] at two levels ( high and low) were used to identify critical process parameters (CPP) and critical material attributes (CMA). The liposomal batches were characterized using Gas chromatography–mass spectrometry (GC-MS) and Malvern Zetasizer. Results Egg phospholipid (99.34mg/g) had significantly higher phosphatidylcholine (PC) than peanut phospholipid (5.56mg/g) and was subsequently used for the liposomal preparations. The total phenol content and the total flavonoid content of the leaf extract, calculated using calibration curves, were 4.67mg salicylic acid equivalent/g and 149.9mg quercetin equivalent/g, respectively. GC-MS data indicated that oleic acid and phytol are the major components present in the leaf extract of Corchorus olitorius. Zetasizer data showed that liposomes obtained were in the range of 109 – 184 nm. Results suggest that injection rate and phospholipid: cholesterol ratio are the most critical variables affecting the particle size distribution of COE liposomes. Batches processed with Poloxamer 188 showed low average size of COE liposomes in its presence. Conclusion Liposomes of desirable nano range were achieved by controlling process parameters and material attributes. Encapsulation efficiency of the extract within the liposomes will be determined and in vitro antibacterial and antitumor activities of the liposomal preparation will be evaluated in further studies.

Journal : Volume : Year : 2017 Issue :
Pages : City : Sandiego, USA Edition : Editors :
Publisher : AAPS annual meeting and exposition , 2017 ISBN : Book : Chapter :
Proceeding Title : American Association of Pharmaceutical scientists (AAPS) Annual Meeting and Exposition, USA. Institution : Issuer : Number :