Title : A Comparative Study of Lipid-Based Nano Drug Delivery System Using MSP1D1 Protein and Poloxamer 407

Authors : S. Subha, M, Mannan, F. Khan, Z.N. Audrika, M. Kazi & M.H. Shariare

Abstract : Purpose: Curcumin is a potent anti-inflammatory and anticancer agent [1]. Due to its poor solubility and low bioavailability, curcumin requires advanced drug delivery systems. Previous studies reported that the membrane scaffolding protein MSP1D1 used in a lipid-based nano drug delivery system provides very small particle size [2]. However, MSP1D1 is expensive, requires specific storage conditions and the process of preparing the nano carrier system using protein is time consuming. Therefore, this study aims to investigate a potential alternative for MSP1D1 to develop a lipid-based nano drug delivery system. Methods: MSP1D1 and Poloxamer 407 were purchased from Sigma-Aldrich (Germany). Curcumin crystalline powder (extra pure) was purchased from Loba Chemie (India). Phospholipids were extracted in-house using chicken eggs. All solvents used were of HPLC grade. The lipid-based nano carrier systems were developed using injection method. Average particle size and zeta potential value of the nano carrier systems were determined using Malvern Zetasizer (without filtration). Entrapment efficiency of curcumin in the nano carrier systems was determined by UV spectrophotometry at 420nm, using acetone as solvent. Results: Malvern Zetasizer results showed that the average particle size for the batches prepared using MSP1D1 ranged from 170-200nm, while batches prepared using Poloxamer were in the range of 150-360nm (Table 1 & 2). The lowest average particle size was observed for the nano carrier batch prepared using poloxamer, is 156nm (Table 2). Malvern Zetasizer results also indicated that zeta potential value was higher for batches prepared with MSP1D1 (Table 1). The low zeta potential value exhibited by the poloxamer batches may be attributed to the non-ionic nature of the stabilizer. Entrapment efficiency of curcumin for all the nano carrier batches was in the range of 35% - 98%. Highest entrapment efficiency was also observed for a batch prepared using poloxamer, which is 98.26%. Process parameters perspective, nano carrier batches prepared using 500rpm and without freeze-thaw exhibited low average particle size and high entrapment efficiency (Table 1 & Table 2). Batches prepared using protein, with or without freeze-thaw, did not show any significant difference between average particle sizes. However, average particle size was significantly large for nano carrier batch prepared with freeze-thaw, when poloxamer was used (Table 1 & Table 2). Material attributes perspective, nano carrier batches prepared using protein and cholate (as surfactant) exhibited high entrapment efficiency compared to batches processed without cholate (Table 1). Batches prepared using poloxamer with cholesterol showed higher entrapment efficiency compared to batches processed without cholesterol at 500rpm (Table 2). Conclusion: The lipid-based nano drug delivery systems developed using poloxamer showed better results in terms of particle size and entrapment efficiency. However, the zeta potential value of these batches were low compared to the batches prepared with MSP1D1, in future the goal is to further investigate the reason behind this and make improvements accordingly

Journal : Volume : Year : 2019 Issue :
Pages : City : Texas Edition : Editors :
Publisher : AAPS annual meeting and exposition , 2019 ISBN : Book : Chapter :
Proceeding Title : American Association of Pharmaceutical scientists (AAPS) Annual Meeting and Exposition, USA. Institution : Issuer : Number :