Title : Toxicogenetic study of omeprazole and the modulatory effects of retinol palmitate and ascorbic acid on Allium cepa

Authors : Braga AL, de Meneses APM, Santos JVO, Dos Reis AC, de Lima RMT, da Mata AMOF, Paz MFCJ, Alves LBDS, Shaw S, Uddin SJ, Rouf R, Das AK, Dev S, Shill MC, Shilpi JA, Khan IN, Islam MT, Ali ES, Mubarak MS, Mishra SK, E Sousa JMC, Melo-Cavalcante AAC.

Abstract : Omeprazole (OME) is a proton pump inhibitor used for the treatment of various gastric and intestinal disease; however, studies on its effects on the genetic materials are still restricted. The present study aimed to evaluate possible toxicogenic effects of OME in Allium cepa meristems with the application of cytogenetic biomarkers for DNA damage, mutagenic, toxic and cytotoxic effects. Additionally, retinol palmitate (RP) and ascorbic acid (AA) were also co-treated with OME to evaluate possible modulatory effects of OME-induced cytogenetic damages. OME was tested at 10, 20 and 40 mg/mL, while RP and AA at 55 mg/mL and 352.2 mg/mL, respectively. Copper sulphate (0.6 mg/mL) and dechlorinated water were used as positive control and negative control, respectively. The results suggest that OME induced genotoxicity and mutagenicity in A. cepa at all tested concentrations. It was noted that cotreatment of OME with the antioxidant vitamins RP and/or AA significantly (p < 0.05) inhibited and/or modulated all toxicogenic damages induced by OME. These observations demonstrate their antigenotoxic, antimutagenic, antitoxic and anticitotoxic effects in A. cepa. This study indicates that application of antioxidants may be useful tools to overcome OME-induced toxic effects.

Journal : Chemosphere Volume : 204 Year : 2018 Issue :
Pages : 220-226 City : Edition : Editors :
Publisher : ISBN : Book : Chapter :
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