Title : In Vitro Plant Regeneration from Shoot Tip Explants Of Jatropha Curcas L: A Biodiesel Plant.


Authors : Kamrun Nahar and Rita Sarah Borna

Abstract : In vitro regeneration was achieved from shoot tip explants of 4 months old Jatropha curcas plant. Propagation from shoot tip was evaluated on a range of concentrations of Benzyl adenine (BA) with indole-3-butyric acid (IBA) as 2.22 ?M/L BA and 4.9?M/L IBA, 1.11 ?M/M BA and 0.48 ?M/L IBA, 0.42 ?M/L BA and 0.46?M/L IBA and 0.44 ?M/L BA and 0.44 ?M/L IBA. Higher regeneration potential that direct adventitious shoot induction was recorded highest on MS medium with 2.22 ?M/L BA and 4.9 ?M/L IBA. Regenerated shoots then rooted on half strength MS medium supplemented with IBA (0.5?M). Following simple hardening procedures, the in vitro raised plants were transferred to soil and grown to maturity in the field.


Journal : ARPN Journal of Science and Technology Volume : 3 Year : 2013 Issue : 1
Pages : 38-42 City : Edition : Editors :
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