NSU Research Contributions
Title : RoleofMuscarinicAcetylcholineReceptorsin SerialFeature-Positive DiscriminationTask duringEyeblinkConditioninginMice
Authors : Md. Ashrafur Rahman, Norifumi Tanaka, Koji Usui, Shigenori Kawahara
Abstract : Weinvestigated theroleofmuscarinicacetylcholinereceptors(mAChRs)ineyeblinkserial feature-positivediscrimination learning inmiceusingthemAChR antagonist.A2-slightcue wasdelivered 5or6sbeforethepresentationofa350-mstonepairedwitha100-msperiorbitalelectricalshock(cuedtrial)butnotbeforethetone-alonepresentation(non-cuedtrial). Micereceived30cuedand30non-cuedtrials eachdayinarandomorder.Wefoundthat saline-injectedcontrolmiceweresuccessfullydiscriminatingbetweencuedandnon-cued trialswithinafewdaysofconditioning.Themicerespondedmorefrequentlytothetone incuedtrialsthaninnon-cuedtrials.Analysisofconditionedresponse(CR)dynamics revealedthattheCRonsetlatencywasshorterincuedtrials thaninnon-cuedtrials,despite theCRpeakamplitudenotdifferingsignificantlybetweenthetwoconditions.Incontrast, scopolamine-injected micedevelopedanequalnumberofCRswithsimilartemporalpatternsirrespectiveofthepresenceofthecueduringthe7daysofconditioning,indicatingin afailuretoacquireconditionaldiscrimination.Inaddition,thescopolamineadministration tothecontrolmiceaftertheyhadsuccessfullyacquireddiscriminationdidnotimpairthe conditionaldiscriminationandexpressionofpre-acquiredCR.Theseresultssuggestthat mAChRsmayplayapivotalroleinmemoryformationintheconditionalbrainstateassociatedwiththefeature cue;howevertheyareunlikelytobeinvolvedinthedevelopmentof discriminationafterconditionalmemoryhadformedintheserialfeature-positivediscriminationtaskduringeyeblinkconditioning.
| Journal : PLOs ONE | Volume : PLoSONE11(1): e0147572. | Year : 2016 | Issue : PLoS ONE 11(1) |
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