NSU Research Contributions
Title : Proteome study unveils the regulation of metabolic pathways during the biofilm formation process in the ?hnox Paracoccus denitrificans
Authors : Aishat Alatishe; Md . Shariful Islam; Erik Yukl
Abstract : Paracoccus denitrificans, a non-motile gram-negative bacterium, is often used as a model organism for studies of denitrification, respiratory chains, and polyhydroxyalkanoate production due to its metabolic versatility. Biofilm in Paracoccus denitrificans, compared to the complex multilayered biofilms observed in other bacterial species, exhibits a distinctive monolayer form. The secondary messenger cyclic-di-GMP (c-di-GMP) has been shown to regulate biofilm formation as elevated levels of c-di-GMP are associated with increased biofilm formation in gram negative bacteria. H-NOXs are a type of hemoprotein sensors found in bacteria that play a crucial role in facilitating collective responses, such as the production of biofilms, in reaction to low levels of nitric oxide (NO). The production of nitric oxide (NO) through the denitrification pathway has been observed to stimulate the formation of biofilms. In the current study, we examined the function of hnox within the framework of P. dentrificans. We demonstrated that the recombinant PdHNOX protein exhibits characteristics of folding and stability. However, it does not show significant binding affinity towards either heme or zinc. The deletion of hnox gene in P. dentrificans indicates a disruptive effect on the production of biofilms. Notably, there was not a noticeable alteration in the growth rate and survival rate when comparing ?hnox and WT P. denitrificans, indicating that the hnox gene, while not exhibiting any discernible impact on features, does indeed exert a notable influence on the formation of biofilms. Moreover, to provide a comprehensive understanding of the influence of the hnox deletion in P. denitrificans on its biofilm generation and to identify its potential targets, we performed a comparative analysis of proteome data derived from ?hnoxP. denitrificans and its WT variant. Our quantitative proteomics study identifies alterations in a total of 104 proteins associated with metabolism, biofilm, and ATP synthase. Furthermore, the current study proposes a total of 24 potential interaction partners of HNOX. To the best of our knowledge, this study represents the most extensive study on proteomics in P. denitrificans conducted so far. In addition, it is the first study in which the involvement of H-NOX in the production of pyruvate-associated biofilms has been hypothesized.
| Journal : | Volume : | Year : 2024 | Issue : |
| Pages : | City : | Edition : | Editors : |
| Publisher : Elsevier | ISBN : | Book : | Chapter : |
| Proceeding Title : The American Society for Biochemistry and Molecular Biology | Institution : | Issuer : | Number : |
