NSU Research Contributions

Title : H-NOX Influences Biofilm Formation, Central Metabolism, and Quorum Sensing in Paracoccus denitrificans

Authors : Md. Shariful Islam, Aishat Alatishe, Cameron C. Lee-Lopez, Fred Serrano, Erik T. Yukl

Abstract : The transition from planktonic to biofilm growth in bacteria is often accompanied by greater resistance to antibiotics and other stressors, as well as distinct alterations in physical traits, genetic activity, and metabolic restructuring. In many species, the heme nitric oxide/oxygen binding proteins (H-NOX) play an important role in this process, although the signaling mechanisms and pathways in which they participate are quite diverse and largely unknown. In Paracoccus denitrificans, deletion of the hnox gene results in a severe biofilm-deficient phenotype. Quantitative proteomics was used to assemble a comprehensive data set of P. denitrificans proteins showing altered abundance of those involved in several important metabolic pathways. Further, decreased levels of pyruvate and elevated levels of C16 homoserine lactone were detected for the ?hnox strain, associating the biofilm deficiency with altered central carbon metabolism and quorum sensing, respectively. These results expand our knowledge of the important role of H-NOX signaling in biofilm formation.

Journal : Journal of Proteome Research	Volume :	Year : 2024	Issue :
Pages :	City :	Edition :	Editors :
Publisher :	ISBN :	Book :	Chapter :
Proceeding Title :	Institution :	Issuer :	Number :