NSU Research Contributions
Title : Vogesella oryzagri sp. Nov., isolated from the rhizosphere of rice and in silico genome mining for the prediction of biosynthetic gene clusters.
Authors : Md. Amdadul Huq, Yeon-Ju Kim, M. Mizanur Rahman, Md. Morshedul Alam, Sathiyaraj Srinivasa, Kwon-Kyoo Kang, Shahina Akter.
Abstract : A Gram-stain-negative, aerobic, rod-shaped, motile and flagellated novel bacterial strain, designated MAHUQ-64T, was isolated from the rhizosphere of rice. The colonies were observed to be creamy white-coloured, smooth, spherical and 0.5–1.1?mm in diameter when grown on Reasoner’s 2A agar medium for 2?days. Strain MAHUQ-64T was able to grow at 10–40?°C, at pH 5.0–9.5 and in the presence of 0–2.0%?NaCl (w/v). The strain was positive for both catalase and oxidase tests. The strain was positive for hydrolysis of l-tyrosine. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Vogesella and is closely related to Vogesella oryzae L3B39T (98.6% sequence similarity) and Vogesella facilis TTM-24T (98.2%). The novel strain MAHUQ-64T has a draft genome size of 3?827?146?bp (22 contigs), annotated with 3612 protein-coding genes, 74 tRNA and 4 rRNA genes. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain MAHUQ-64T and its closest member V. oryzae L3B39T were 86.5 and 33.4%, respectively. In silico genome mining revealed several biosynthetic gene clusters in the genome of the novel strain MAHUQ-64T. The genomic DNA G+C content was determined to be 63.4 mol%. The predominant isoprenoid quinone was ubiquinone-8. The major fatty acids were identified as summed feature 3 (comprising C16??:??1?7c and/or C16??:??1?6c) and C16??:??0. Based on dDDH, ANI value, genotypic analysis and chemotaxonomic and physiological data, strain MAHUQ-64T represents a novel species within the genus Vogesella, for which the name Vogesella oryzagri sp. nov. is proposed, with MAHUQ-64T (=KACC 22245T=CGMCC 1.19000T) as the type strain.
| Journal : International Journal of Systematic and Evolutionary Microbiology | Volume : 75 | Year : 2025 | Issue : 2 |
| Pages : 006687 | City : | Edition : | Editors : |
| Publisher : | ISBN : | Book : | Chapter : |
| Proceeding Title : | Institution : | Issuer : | Number : |
